Active Transport of γ-Aminobutyric Acid in Brain Cortex Slices, with Special Reference to Phosphorus-32 Turnover of Phospholipids in Cytoplasmic Particulates

Abstract

WHEN slices of guinea pig brain cortex were incubated aerobically at 37° C. in a saline medium containing amino-acid such as γ-aminobutyric acid or glutamate, the amino-acid was accumulated in the brain slices actively from the medium against its concentration gradient in the presence of glucose as a substrate. Terner et al. ¹ reported that L-glutamate accumulated in brain slices accompanied by potassium ions when they were incubated aerobically in the medium containing glucose. Also as reported previously², non-metabolizable D-isomer of glutamic acid accumulated in the brain slices in the same manner.