Abstract
FRACTIONATION of erythrocytes of different ages by differential hæmolysis is a useful approach to the study of enzymatic aspects of the maturation of the erythrocyte in the peripheral blood. Young red cells are thought to be resistant to osmotic hæmolysis and older cells to be very fragile1. Recently Simon and Topper2 have shown by serial osmotic hæmolysis that young erythrocytes have both fragile and resistant components. In the present paper the activities of glutamic oxaloacetic transaminase and lactic dehydrogenase have been related to red blood cells of various ages.
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References
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Simon, E. R., and Topper, Y. J., Nature, 180, 1211 (1957).
This use of sulphur-35 amino-acids was validated in a group of rats by periodic bleedings. The resulting curve gave a mean survival-time of the erythrocyte as 55 days, consistent with other estimates; compare Berlin, N. I., Van Dyke, D. C., and Lotz, C., Proc. Soc. Exp. Biol. Med., 82, 287 (1953). Burwell, E. L., Brickley, B. A., and Finch, C. A., Amer. J. Physiol., 172, 718 (1953). Belcher, E. H., and Hariss, E. B., J. Physiol., 146, 217 (1959).
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Borsook, H., Deasy, C. L., Haagen-Smit, A. J., Keighley, G., and Lowy, P. H., J. Biol. Chem., 196, 669 (1952). Rubinstein, D., Ottolenghi, P., and Denstedt, P. F., Can. J. Biochem. Physiol., 34, 222 (1956). Marks, P. A., Johnson, A. B., and Hirschberg, E., Proc. U.S. Nat. Acad. Sci., 44, 525 (1958).
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LEVY, L., WALTER, H. & SASS, M. Enzymes and Radioactivity in Erythrocytes of Different Ages. Nature 184, 643–644 (1959). https://doi.org/10.1038/184643a0
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DOI: https://doi.org/10.1038/184643a0
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