Abstract
IN the first report1,2, on ‘crossing paper electrophoresis’, a direct demonstration of enzyme-substrate complex has been described. The enzymes tested included crystallized pure trypsin, chymotrypsin and ribonuclease.
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References
Nakamura, S., Takeo, K., Tanaka, K., and Ueta, T., Z. Physiol. Chem., (in the press).
Nakamura, S., Hosada, T., and Ueta, T., Proc. Japan Acad., 34, 742 (1958).
Thomas, K. (personal communication).
Chance, B., in “Currents in Biochemical Research”, ed. D. E. Green, 308 (Interscience Pub., New York, 1956).
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NAKAMURA, S., TAKEO, K., SASAKI, I. et al. Proof of the Formation of Enzyme-Substrate Complex by ‘Crossing-Paper Electrophoresis’. Nature 184, 638–639 (1959). https://doi.org/10.1038/184638a0
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DOI: https://doi.org/10.1038/184638a0
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