Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Production of Serum Albumin and of Globulins by Chick Mesenchymal Tissue and by HeLa Tumour Tissue in Culture

Nature volume 184, pages 361–362 (1959)Cite this article

An Erratum to this article was published on 26 September 1959

Abstract

A TECHNIQUE has been described for establishing the autonomous production of well-defined soluble proteins by tissue in culture1,2. The tissue is grown in a medium containing a radioactive amino-acid. After incubation, the tissue is homogenized with the medium, the homogenate centrifuged, inactive amino-acid added as a hold back carrier, and the proteins in the supernatant separated from all compounds of low molecular weight, including the radioactive amino-acid, by ultrafiltration under pressure (nitrogen). The different proteins are then separated, burnt to carbon dioxide, and the radioactivity of the carbon is determined with the very sensitive gas Geiger counter3,4. Radioactivity of the carbon dioxide indicates that the amino-acid has been incorporated into the protein, that is, that proteins have been synthesized from the amino-acids by the tissue.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Manner, G., Broda, E., and Kellner, G., Monatsh. Chem., 88, 896 (1957).

    Article  CAS  Google Scholar 

  2. Broda, E., Suschny, O., and Kellner, G., Conference on the Peaceful Uses of Atomic Energy, Geneva, 1958, Report No. 1438.

  3. Broda, E., and Rohringer, G., Z. Elektrochem., 58, 634 (1954).

    CAS  Google Scholar 

  4. Broda, E., J. Inorg. Nucl. Chem., 1, 412 (1955).

    Article  Google Scholar 

  5. Dulbecco, R., and Vogt, M., J. Exp. Med., 99, 167 (1954).

    Article  CAS  Google Scholar 

  6. Kellner, G., and Stockinger, L., Arch. Intern. Pharmacodynamie, 110, 259 (1957).

    CAS  Google Scholar 

  7. Cohn, E. J., Strong, L. E., Hughes, W. L., Mulford, D. J., Ashworth, J. N., Melin, M., and Taylor, H. L., J. Amer. Chem. Soc., 68, 459 (1946).

    Article  CAS  Google Scholar 

  8. Smithies, O., Biochem. J., 61, 629 (1955).

    Article  CAS  Google Scholar 

  9. Winnick, T., Arch. Biochem. Biophys., 27, 65 (1950).

    CAS  Google Scholar 

  10. Landsteiner, K., and Parker, R. C., J. Exp. Med., 71, 231 (1940).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. First Chemical Institute,

    Y. ABDEL-SAMIE, E. BRODA, G. KELLNER & W. ZISCHKA

  2. Department of Histology and Embryology, University, Vienna,

    Y. ABDEL-SAMIE, E. BRODA, G. KELLNER & W. ZISCHKA

Authors
  1. Y. ABDEL-SAMIE
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. BRODA
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. KELLNER
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. W. ZISCHKA
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

ABDEL-SAMIE, Y., BRODA, E., KELLNER, G. et al. Production of Serum Albumin and of Globulins by Chick Mesenchymal Tissue and by HeLa Tumour Tissue in Culture. Nature 184, 361–362 (1959). https://doi.org/10.1038/184361a0

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/184361a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing