Abstract
WHILE investigating the changes which occur in plant cytoplasmic proteins during growth and differentiation, using the Ouchterlony serum – agar plate technique1, the need arose for a quantitative method of estimating the protein constituents. Although there are several methods available2–7, none of them is easily applicable to a multi-antigenic system, and in addition the concentrations of the proteins can only be estimated separately, even under favourable conditions, at ±10 per cent and more usually ±50 per cent.
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References
Ouchterlony, O., Acta Path. Microbiol. Scand., 25, 186 (1948).
Becker, E. L., Munoz, J., Lapresle, C., and Le Beau, L. J., J. Immunol., 67, 501 (1951).
Oudin, J., “Methods in Medical Research”, 5, 335 (Year Book Publishers, Chicago, 1952).
Oakley, C. L., and Fulthorpe, A. J., J. Path. Bact., 65, 49 (1953).
Augustin, R., and Hayward, B. J., Int. Arch. Allergy, 6, 154 (1955).
Feinberg, J. G., Nature, 177, 531 (1956).
Gell, P. G. H., J. Clin. Path., 10, 67 (1957).
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WRIGHT, S. A Quantitative Serum–Agar Technique. Nature 183, 1282–1283 (1959). https://doi.org/10.1038/1831282b0
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DOI: https://doi.org/10.1038/1831282b0
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