Lysins and Agglutinins in Normal and Lymphomatous Mouse Tissue

Abstract

LYSINS and agglutinins have been extracted from normal mouse tissues, and often in much higher concentration from the mammary adenocareinomas of the female C3H mouse. Three methods have been used for extraction^1–4: (a) extraction of minced-up tissues in saline (1 per cent sodium chloride), (b) ‘pre-incubation’ of homogenized tissue at 37° C. in saline for 12–18 hr., which results in the separation of a yellow hæmolytic fluid, and (c) extraction with ether, which gives an ether-soluble ‘soap-like’ lytic material and also a lytic material insoluble in cold ether (‘lysolceithin’). These methods and their results have been reviewed by Ponder³ in relation to a background which begins with the work of Metchnikoff in 1893. Probably only method (a) is of real value for demonstrating tissue lysins and inhibitors; the other two methods are to some extent unreliable since they involve the splitting of pre-existing complexes.