Bacterial Preparation of Orotidine-5′-phosphate and Uridine-5′-phosphate

Abstract

THE enzymic syntheses of orotidine-5′-phosphate and uridine-5′-phosphate have been reported by Lieberman et al. ¹ and others. A chemical phosphorylation of uridine to form the 5′-phosphates has also been described by Hall and Khorana². This communication describes the preparation of these previously rare compounds from cultures of E. coli B grown in a salts–glucose medium in the presence of the uracil analogue, as-triazine-3,5-dione, referred to as 6-azauracil.