Abstract
RECENTLY, we showed that the quantitative analysis of amino-acids using the maximum colour density method could be accomplished with low errors and good reproducibility1. As regards the analysis of hydroxyproline, the accuracy can be considerably improved if the colour development is effected using a ninhydrin–isatin–triethylamine reagent2 rather than isatin1.
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Roberts, H. R., and Kolor, M. G., Anal. Chem., 29, 1800 (1957).
Kolor, M. G., and Roberts, H. R., Arch. Biochem. Biophys., 70, 620 (1957).
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ROBERTS, H., KOLOR, M. Quantitative Determination of Hydroxyproline by Paper Chromatography. Nature 181, 837 (1958). https://doi.org/10.1038/181837a0
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DOI: https://doi.org/10.1038/181837a0
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