Abstract
DURING investigations in which sudan black B was used for studying the lipoid inclusions in cells of bone and teeth1, it was found that dentin, enamel, cartilage and bone did not stain significantly unless they were previously extracted with pyridine at 60° C., when characteristic staining reactions were obtained.
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References
Irving, J. T., Nature, 181, 569 (1958).
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Scott, B. L., and Pease, D. C., Anat. Rec., 126, 465 (1957).
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IRVING, J. A Histological Stain for Newly Calcified Tissues. Nature 181, 704–705 (1958). https://doi.org/10.1038/181704a0
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DOI: https://doi.org/10.1038/181704a0
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