Abstract
RECENTLY, it has been obvious that in most biological materials N-acetylsialic acid (N-acetyl-neuraminic acid) and N-glycolloylsialic acid occur together. By the introduction of a micromethod for the determination of glycollic acid, Klenk and Uhlenbruck1 have made possible the evaluation of the amount of N-glycolloylsialic acid in isolated sialic acids. In search of a more direct method, we tried partition chromatography, employing the solvent systems previously used for the separation of sialic acids but with unfavourable results2,3. As the pK a of sialic acids are low, about pH 2.7, it is necessary to use rather a strong acid in the solvents to prevent the dissociation of the carboxylic group. Of the different acids tried, only trichloracetic acid and hydrochloric acid were suitable. On crystallization of the sialic acids we had observed that N-glycolloylsialic acid was much more insoluble in alcohols than N-acetylsialic acid. Therefore, we tried mixtures of alcohols with the acids mentioned above. The best results were obtained with n-butanol/n-propanol/ 0.1 N hydrochloric acid (1:2:1, v/v). In this solvent the R F values were for N-acetylsialic acid 0.45, and for N-glycolloylsialic acid 0.34, on Whatman paper No. 1. The solvent system applied cannot be used for the separation of diacetylsialic acids and is unsuitable for the separation of free acids and the methyl esters of the sialic acids.
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References
Klenk, E., and Uhlenbruck, G., Z. physiol. Chem., 307, 266 (1957).
Blix, G., Lindberg, E., Odin, L., and Werner, I., Acta Soc. Med. Upsal., 61, 1 (1956).
Svennerholm, L., Acta Soc. Med. Upsal., 61, 75 (1956).
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SVENNERHOLM, E., SVENNERHOLM, L. Quantitative Paper Partition Chromatography of Sialic Acids. Nature 181, 1154–1155 (1958). https://doi.org/10.1038/1811154a0
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DOI: https://doi.org/10.1038/1811154a0
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