Abstract
ABOUT ten years ago Keston and co-workers developed an isotope dilution method for the estimation of amino-acids1. This method, by which the amino-group of amino-acids is labelled with radio-active ‘pipsyl chloride’ (p-iodophenylsulphonyl chloride, labelled with iodine-131), was not found applicable for other types of compounds until recently. Last year, however, this method was also used for the detection and estimation of histamine2, pyrimidines3 and 17-hydroxycorticosterone4. In the last method the labelling agent was ‘pipsan’ (p-iodophenylsulphonylic acid anhydride).
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References
Keston, A. S., Udenfriend, S., and Cannan, R. Keith, J. Amer. Chem. Soc., 71, 249 (1949).
Schayer, R. W., Kobayashi, Yutaka and Smiley, R. L., J. Biol. Chem., 212, 593 (1955).
Fresco, J. R., and Warner, R. C., J. Biol. Chem., 215, 751 (1955).
Bojesen, E., Scand. J. Clin. and Lab. Invest., 8, 55 (1956).
Keston, A. S., Udenfriend, S., and Levy, M., J. Amer. Chem. Soc., 72, 748 (1950).
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LEEGWATER, D. An Isotope-Derivative Method for the Detection and Estimation of Œstrogens. Nature 178, 916–917 (1956). https://doi.org/10.1038/178916a0
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DOI: https://doi.org/10.1038/178916a0
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