Abstract
DURING the course of routine examination of strain SU298 (Sydney University strain) of Rhizobium trifolii, it was suspected that the strain was carrying a bacteriophage. Subsequent investigation proved strain SU298 to be lysogenic, and the closely related strain SU297 to be a susceptible indicator strain. The field-performances and relationships of these strains have recently been reviewed by Vincent1. The occurrence of lysogeny in SU298 was demonstrated by seeding yeast mannitol agar (0.4 per cent agar) with appropriate proportions of each strain and incubating the plates at 26° C. After 24–48 hr., plaques could be observed in the otherwise confluent growth of SU297. At the centre of each plaque was a colony of the lysogenic strain. The lysogenic nature of strain SU298 was confirmed by the fact that it proved to be resistant to the purified phage ; strain SU297, on the other hand, was fully susceptible.
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References
Vincent, J. M., Proc. Linn. Soc., N.S.W., 79, iv (1954).
Marshall, K. C., and Vincent, J. M., Aust. J. Sci., 17, 68 (1954).
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MARSHALL, K. A Lysogenic Strain of Rhizobium trifolii . Nature 177, 92 (1956). https://doi.org/10.1038/177092a0
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DOI: https://doi.org/10.1038/177092a0
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