Abstract
WHEN bull semen is diluted in a buffered (pH. 7.0) solution of sodium chloride which is isotonic with bull seminal plasma (Δ, − 0.54° C.) and is filtered through a layer of small glass balls, the dead spermatozoa are retained by the filter while live ones pass through; it is therefore possible to estimate the percentage dead spermatozoa in bull semen by comparing the opacity of diluted semen before and after filtration.
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Campbell, R. C., Hancock, J. L., and Rothschild, Lord, J. Exp. Biol., 30, 44 (1953).
Bishop, M. W. H., Campbell, R. C., Hancock, J. L., and Walton, A., J. Agric. Sci., 44, 227 (1954).
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BANGHAM, A., HANCOCK, J. A New Method for counting Live and Dead Bull Spermatozoa. Nature 176, 656 (1955). https://doi.org/10.1038/176656a0
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DOI: https://doi.org/10.1038/176656a0
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