Abstract
THE importance of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) in the physiology of a cell is very great. Very little work on cells of fungal hyphæ seems to have been done. For this purpose, very young (2–3 days old) subcultures of Polyporus ostreiformis, Polyporus adustus, Polyporus gilvus and Marasmius campanella were taken; they were fixed and stained for 15–20 min. by the method of Unna as adapted by Brachet1. The small resting nuclei of the homogeneous unexpanded type of Pinto Lopes2 became very prominent in pyronin-stained cytoplasm; nucleolus and chromatin, combined at the centre into a homogeneous mass, took brilliant green stain (DNA) surrounded by pink hyloplasm (RNA), and finally by a green outline (Fig. 1). The nucleolus, according bo Brachet3 and Caspersson4, is believed to be composed mainly of ribonucleoproteins, and Fujii5 holds that zinc, in addition, is present in nucleoli and that, upon the initiation of mitosis, zinc passes into chromosomes and possibly also into prometaphase spindle.
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BOSE, S. Deoxyribonucleic Acid and Ribonucleic Acid in Hyphal Cells of Higher Fungi. Nature 175, 735 (1955). https://doi.org/10.1038/175735a0
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DOI: https://doi.org/10.1038/175735a0
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