Abstract
WE have been studying the concurrent synthesis in vitro in rabbit reticulocytes of hæm, globin and the non-hæmoglobin proteins. Using methylene-labelled (14C) glycine the degree of ribonucleic acid synthesis also could be measured. The technique devised may be useful to others faced with the problem of measuring the radioactivity of very small amounts of substances in very dilute solution. It has enabled us to observe the synthesis of five nucleotide components of ribonucleic acid, beginning with 8 ml. of reticulocytes.
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References
Borsook, H., Abrams, A., and Lowy, P. H., J. Biol. Chem. (submitted for publication).
Borsook, H., Deasy, C. L., Haagen-Smit, A. J., Keighley, G., and Lowy, P. H., J. Biol. Chem., 196, 669 (1952).
Loring, H. S. (personal communication).
Cohn, W. E., J. Amer. Chem. Soc., 72, 1471 (1950).
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KRUH, J., BORSOOK, H. In vitro Synthesis of Ribonucleic Acid in Reticulocytes. Nature 175, 386–387 (1955). https://doi.org/10.1038/175386b0
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DOI: https://doi.org/10.1038/175386b0
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