Abstract
IN the preparation of aminofluorescein by the condensation of 4-nitrophthalic acid and rosorcinol followed by reduction, two isomers (A) and (B) are formed at the condensation stage, and those must be separated if unequivocal results are to be obtained in the subsequent reaction of the derived fluorescein isocyanate with proteins to give fluorescent protein conjugates1.
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References
Coons, A. H., and Kaplan, M. H., J. Exp. Med., 91, 1 (1950).
Donaldson, K. O., Tulane, V. J., and Marshall, L. M., Anal. Chem., 24, 185 (1952).
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DE REPENTIGNY, J., JAMES, A. A Chromatographic Separation of the Aminofluorescein Isomers. Nature 174, 927–928 (1954). https://doi.org/10.1038/174927b0
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DOI: https://doi.org/10.1038/174927b0
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