Phase-Contrast Microscopy of the Gomori Reaction for Alkaline Phosphatase

Abstract

IN the standard Gomori technique for the histochemical demonstration of alkaline phosphatase, calcium phosphate deposits are formed in sections supposedly at sites of enzyme activity. These deposits, thought to be invisible, must be brought to light by a further procedure. Calcium may be converted to cobalt and the latter visualized as the black sulphide; the von Kossa silver technique may be employed; or lake-forming dyes may be used for colouring the deposits.