Abstract
WE have already reported the partial purification from sow's milk and desiccated pig stomach of proteins which combine with cyanocobalamin and the vitamin B12-like substances having different nucleotide groups1. Purification of the protein from sow's milk whey was achieved by continuous electro-phoresis on paper2. Because of the labile nature of the protein itself, we found it easier to saturate the impure concentrate with cyanocobalamin labelled with cobalt-60 and to isolate the cyanocobalamin–protein complex. Purification of this complex by continuous electrophoresis on paper2 was followed by repeated fractionation with isopropyl alcohol at −5°. A recent preliminary note by Wijmenga, Thompson, Stern and O'Connell3, concerning a similar cyanocobalamin–protein from pig gastric mucosa, has prompted us to give a brief account of the properties of our substance isolated from sow's milk. Full experimental details will be published elsewhere.
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GREGORY, M., HOLDSWORTH, E. A Cyanocobalamin–Protein Complex from Sow's Milk and Desiccated Pig Stomach. Nature 173, 830 (1954). https://doi.org/10.1038/173830a0
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DOI: https://doi.org/10.1038/173830a0
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