Abstract
IT was recently suggested that a ‘new’ amino-acid isolated from the apple fruit was a methyl proline1. This acid (called D 1 in the original communication) has now been compared with an authentic specimen of γ-methyl proline kindly supplied by Prof. F. C. Steward, of Cornell University. The positions occupied by these two acids on two-dimensional chromatograms (phenol ammonia–butanol–acetic acid–water) were found to be identical. Mr. J. K. Pollard in Prof. Steward's laboratory has also found the two compounds to superimpose on collidine–lutidine: phenol chromatograms. The two compounds (D 1 and γ-methyl proline) give identical colour reactions on paper chromatograms with ninhydrin (a yellow-orange colour) and with isatin (a pure blue colour developing at the same rate with both acids). Not more than ∼ 17 mgm. of D 1 were obtained from 70 kgm. of young Worcester Pearmain apples, and elementary analysis on a sample containing traces of inorganic salt gave the following: C, 53.6 per cent; H, 8.17 per cent ; N, 9.7 per cent. Assuming this indicates a C6 compound with an empirical formula of C6H11O2N, and correcting the analyses for ash gives: C, 55.8; H, 8.5; N, 10.1; methyl proline requires : C, 55.8; H, 8.5; N, 10.8 per cent.
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References
Hulme, A. C., and Arthington, W., Nature, 170, 659 (1952).
Grassman, W., and v.Arnim, K., Liebigs Ann., 519, 192 (1935).
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HULME, A., ARTHINGTON, W. Methyl Proline in Young Apple Fruits. Nature 173, 588–589 (1954). https://doi.org/10.1038/173588b0
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DOI: https://doi.org/10.1038/173588b0
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