Abstract
IN our studies on the synthesis of glutathione by Saccharomyces cerevisiae, the yeast was incubated in 0.01 M potassium dihydrogen phosphate with cysteine containing sulphur-35. Samples of the mixture were removed at intervals and the component amino-acids and peptides were separated by paper chromatography. The chromatograms were sprayed with a ninhydrin solution and autoradiograms of the chromatograms were made on Kodak No-screen X-ray film.
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References
Toennies, G., and Kolb, J. J., Anal. Chem., 23, 823 (1951).
Wikberg, Eskil, Nature, 172, 398 (1953).
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LIVERMORE, A., MUECKE, E. Formation of a Mixed Disulphide of Cysteine and Glutathione. Nature 173, 265–266 (1954). https://doi.org/10.1038/173265b0
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DOI: https://doi.org/10.1038/173265b0
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