Abstract
URINE (210 ml.) from a tuberculous patient who received daily 200 mgm. of isonicotinyl hydrazide was concentrated under diminished pressure to a syrup and was desalted by treatment with absolute alcohol. After evaporation of the solvent, the residual syrup was chromatographed on No. 50 Tōyō filter paper with water-saturated n-butanol as the solvent system, and the spots or the bands were observed under ultra-violet light (2537 A.). The bands which showed R F values of 0.65 (A) and 0.74 (B) (the shadow of A was much stronger than that of B) were cut out, and the elutes from them were again tested by paper chromatography using several solvent systems as indicated in the accompanying table. This showed that A is 1-isonicotinyl-2-acetyl hydrazine whereas B is the unchanged isonicotinyl hydrazide. Confirmation was obtained by investigation of the ultraviolet absorption spectra of fraction A, which was first purified, by development on filter paper with 1 per cent ammonia—iso-propanol and M/50 phosphate buffer - saturated n-butanol system; fraction A has the same absorption curve as that of an authentic sample of 1-isonicotinyl-2-acetyl hydrazine, as indicated in the accompanying figure.
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MAKINO, K., KINOSHITA, T. & ITOH, T. Biological Acetylation of isoNicotinyl Hydrazide. Nature 173, 36 (1954). https://doi.org/10.1038/173036a0
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DOI: https://doi.org/10.1038/173036a0
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