Abstract
A PREVIOUS investigation1 has shown that enzymes from Chœtomium globosum are able to hydrolyse wheat straw xylan to xylose with the formation of a series of intermediates, designated a,b,c, … g, which appear on paper-chromatograms with regularly decreasing RF-values, and which are believed to be oligosaccharides with regularly increasing xylose units: xylobiose2, xylotriose, xylotetraose …etc. By heating or storing the enzyme preparations, it was possible to arrest the hydrolysis at the xylobiose stage, indicating that two enzymes are present, (A) hydrolysing the xyloside chain to xylobiose and (B) hydrolysing xylobiose to xylose.
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References
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Flood, A. E., Hirst, E. L., and Jones, J. K. N., Nature, 160, 86 (1947).
Somogyi, M., J. Biol. Chem., 160, 61 (1945).
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SØRENSEN, H. Enzymatic Hydrolysis of Xylan. Nature 172, 305–306 (1953). https://doi.org/10.1038/172305a0
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DOI: https://doi.org/10.1038/172305a0
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