Abstract
WE have already reported that acetone extracts of liver contain a factor that stimulates the growth of Saccharomyces cerevisiœ1, and of rats2. Liver dried at 110° and finely powdered is refluxed with dry acetone. The acetone extract is evaporated to dry-ness and the residue re-extracted with water. The growth-factor is thus obtained as a clear yellow solution having a pH of 4–5. It is heat-stable, dialysable and is not destroyed by acid or alkali. Part of inactive coloured impurities may be eliminated by passing the extract through a column of activated alumina or animal charcoal. The filtrate is still active and does not give the biuret or ninhydrin reaction.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Tria, E., and Barnabei, O., Boll. Soc. It. Biol. Sper., 27, 133 (1951); Annali Universita' Ferrara, sez. VI, 1 (1952).
Tria, E., and Barnabei, O., Boll. Soc. It. Biol. Sper. (in the press).
Györgyi, P., “Vitamin Methods”, 2, 239 (Academic Press, New York, 1952).
Yudkin, J., Brit. Med. J., i, 1388 (1952).
Dietrich, L. S., Monson, W. J., and Elvehjem, C. A., Arch. Biochem et Biophys., 38, 91 (1952).
Brockman, jun., J. A., Stokstand, E. L. R., Patterson, E. L., Pierce, J. V., Macchi, M., and Day, F. P., J. Amer. Chem. Soc., 74, 1868 (1952).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
TRIA, E., BARNABEI, O. An Unidentified Growth-Factor for Yeast and Hyperthyroid Rats. Nature 172, 203–204 (1953). https://doi.org/10.1038/172203a0
Issue Date:
DOI: https://doi.org/10.1038/172203a0
This article is cited by
-
Karyotypes of Malayan rats (Rodentia-Muridae, genus Rattus Fischer)
Chromosoma (1969)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
