Abstract
IN preparing hydrolysates of polysaccharides for paper chromatography, it has always been considered necessary to neutralize the sulphuric acid with barium carbonate before applying the solution to the paper, as the acid causes disturbing stains. Indeed, when using an ammoniacal silver spray, white stains appear at the place where the spots of glucose and galactose should be; but the flow of the sugars does not seem to be disturbed.
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References
Somogyi, M., J. Biol. Chem., 195, 19 (1952). Nelson, N., J. Biol. Chem., 153, 375 (1944).
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GAILLARD, B. Use of Unneutralized Hydrolysates in Paper Chromatography of Sugars. Nature 171, 1160 (1953). https://doi.org/10.1038/1711160a0
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DOI: https://doi.org/10.1038/1711160a0
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