Abstract
A METHOD has been developed for the assay of progesterone by which a little as 4 µgm. of the hormone can be detected. It is based on the technique of extraction and partition between organic solvents1 and subsequent separation and semi-quantitative estimation by chromatographic partition on filter paper2. The method has been used for the detection and semi-quantitative determination of progesterone in biological material. The results are summarized as follows.
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References
Butt, W. R., Morris, P., Morris, C. J. O. R., and Williams, D. C., Biochem. J., 49, 434 (1951).
Bush, I. E., Biochem. J., 50, 370 (1952).
Hooker, C. W., and Forbes, T. R., Endocrin., 44, 61 (1949).
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EDGAR, D. Progesterone in Body Fluids. Nature 170, 543–544 (1952). https://doi.org/10.1038/170543b0
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DOI: https://doi.org/10.1038/170543b0
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