Stimulating Effect of Amino-Acids on the Survival at 37° C. of Trypanosoma rhodesiense in a Serum-free Synthetic Medium

Abstract

BLOOD (whole, or as plasma, serum, erythrocytes or laked erythrocyte solution) is an essential component of culture media for many pathogenic Protozoa. The essential nutritive components in rabbit, ox, sheep, horse or pig serum required to maintain pathogenic African trypanosomes in culture at 37° C. in the trypanosoma form have not been investigated extensively since the discovery by Yorke, Adams and Murgatroyd¹ that T. equiperdum, T. rhodesiense and T. gambiense could be maintained under these conditions in an actively motile state and in undiminished numbers for 24 hr.