Abstract
THE two essentials for successful tissue culture are a sufficient supply of nutrients and adequate aeration. In the culture of plant embryos from an early stage of development it is also often desirable that cultures shall be suitable for microscopical examination.
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White, P. R., “A Handbook of Plant Tissue Culture” (1943).
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STOKES, P. A New Technique for the Sterile Culture of Plant Embryos in vitro. Nature 170, 242 (1952). https://doi.org/10.1038/170242a0
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DOI: https://doi.org/10.1038/170242a0
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