Abstract
DURING the past year there have been several observations which have indicated that adrenotropic hormone (ACTH), as measured by the ascorbic acid depletion test1, might be a basic substance. Payne, Raben and Astwood2 observed that its activity could be adsorbed on powdered cellulose from dilute acetic acid solution and eluted again by 0.1 N hydrochloric acid, and these experiments agreed with results from preliminary trials in this laboratory on the behaviour of adrenotropic hormone when subjected to solvent partition (some of these initial experiments were carried out in collaboration with Dr. H. G. Khorana, of the Department of Chemistry, University of Cambridge) and preparative electrophoresis. Astwood, Raben, Payne and Grady3 have since utilized a single-step adsorption of adrenotropic hormone activity on oxycellulose (10.4 per cent COOH). We have concurrently been exploring its behaviour on columns of ‘Amberlite IEC-50’, which have previously been found to permit effective chromatographic purification of low-molecular weight basic proteins, such as ribonuclease4 and lysozyme5.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Sayers, M., Sayers, G., and Woodbury, L. A., Endocrin., 42, 379 (1948).
Payne, R. W., Raben, M. S., and Astwood, E. B., J. Biol. Chem., 187, 719 (1950).
Astwood, E. B., Raben, M. S., Payne, R. W., and Grady, A. B., J. Amer. Chem. Soc., 73, 2969 (1951).
Hirs, C. H. W., Stein, W. H., and Moore, S., J. Amer. Chem. Soc., 73, 1893 (1951).
Tallan, H. H., and Stein, W. H., J. Amer. Chem. Soc., 73, 2976 (1951).
Fishman, J. B., J. Biol. Chem., 167, 425 (1947).
Moore, S., and Stein, W. H., J. Biol. Chem., 192, 663 (1951).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
DIXON, H., MOORE, S., STACK-DUNNE, M. et al. Chromatography of Adrenotropic Hormone on Ion-exchange Columns. Nature 168, 1044–1045 (1951). https://doi.org/10.1038/1681044a0
Issue Date:
DOI: https://doi.org/10.1038/1681044a0
This article is cited by
-
Hormonal Stimulation of Melanogenesis in Tissue Culture**From the Department of Dermatology (Clarence S. Livingood, M.D., Director), Henry Ford Hospital; and the Department of Biology (Contribution Number 43), Wayne State University, Detroit, Michigan.This investigation was supported in part by a research grant CY-3345(C1) from the National Institutes of Health, Public Health Service.
Journal of Investigative Dermatology (1960)
-
Separation of Neutral Proteins on Ion-Exchange Resins
Nature (1953)
-
Influence of Adrenotropic Hormone Fractions on ‘Adrenal Repair’ and on Adrenal Ascorbic Acid
Nature (1951)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
