Abstract
PHENOLIC spraying reagents for the detection of sugars on paper chromatograms as introduced by Forsyth1, while greatly improving the selectivity of the method, had the disadvantages that the mineral acid attacked the paper, making it brittle when dried, and giving rise to dark background colours, and that the solvent caused diffusion of the sugar, with consequent poor definition of the spot boundary. Partridge2 found reduced attack on the paper by the use of trichloracetic acid as acidifying agent, and reported improved spot definition with aniline hydrogen phthalate dissolved in butanol3. Other agents proposed have included aniline hydrogen oxalate4, aniline – trichloracetic acid5, benzidine – acetic acid6, and ethanolic solutions of phenols and amines acidified with small amounts of hydrochloric acid7.
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References
Forsyth, W. G. C., Nature, 161, 239 (1946).
Partridge, S. M., Biochem. J., 42, 238 (1948).
Partridge, S. M., Nature, 164, 443 (1949).
Horrocks, R. H., and Manning, G. B., Lancet, 256, 1042 (1949).
Hirst, E. L., and Jones, J. K. N., Discuss. Farad. Soc., No. 7, 268 (1949).
Horrocks, R. H., Nature, 164, 444 (1949).
Novellie, L., Nature, 166, 745 (1950).
de Whalley, H. C. S., Inter. Sugar J., 52, 127, 151 (1950).
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BRYSON, J., MITCHELL, T. Improved Spraying Reagents for the Detection of Sugars on Paper Chromatograms. Nature 167, 864 (1951). https://doi.org/10.1038/167864a0
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DOI: https://doi.org/10.1038/167864a0
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