Abstract
AN investigation has been made on the carbohydrate composition of worts prepared by the infusion and decoction methods of mashing. Although the presence of the sugars, maltose, sucrose, glucose and fructose in wort has long been established, no reliable method for the direct demonstration of these and other sugars has been available in the past. The discovery of paper partition chromatography of carbohydrates1 and its application2 to the quantitative estimation of carbohydrates produced, for example, by the hydrolysis of plant gums and mucilages was considered by us to be an ideal tool for the rapid identification and quantitative estimation of the carbohydrates in wort. The method for the quantitative determination of the sugars of wort was adapted from that of Flood, Hirst and Jones2. This proved to be reliable within limits of ± 10 per cent for any particular sugar percentage and, with practice, a consistent error of not more than ± 5 per cent is obtainable.
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References
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BARTON-WRIGHT, E., HARRIS, G. Carbohydrate Composition of Wort and the Biochemistry of Fermentation. Nature 167, 560–561 (1951). https://doi.org/10.1038/167560a0
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DOI: https://doi.org/10.1038/167560a0
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