Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Colour-Contrast Phase Microscopy

Abstract

THE possibilities of utilizing the differential chromatic effects of the Rheinberg ring method of illumination, which was later developed into the optical staining principles of Kraft1, in conjunction with phase-contrast microscopy, suggested that, instead of having the substage stop quite blacked out except for the annulus, cross or line, whichever the case may be, this should be replaced by an appropriate colour filter, and the portion which usually transmits the light be of another colour in contrast to it.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Kraft, P., Z. deut. geol. Gesell., 84, 651 (1932).

    Google Scholar 

  2. Semmens, C. S., and Bhaduri, P. N., Stain. Tech., 14, 1 (1939).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

GRIGG, F. Colour-Contrast Phase Microscopy. Nature 165, 368–369 (1950). https://doi.org/10.1038/165368b0

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/165368b0

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing