Abstract
THE ‘cutting-out’ technique described by Consden, Gordon and Martin1, and Dent2, for isolating a ninhydrin reacting substance, was applied to the study of these substances in urine. Twelve 20 μl. drops of urine were run side by side on Whatman No. 1 filter paper with phenol–ammonia–hydrogen cyanide. After developing the outer runs with ninhydrin, transverse strips were cut isolating the ninhydrin positive and negative areas. Chromatography of the hydrolysed eluate of each strip (including the negative ones) revealed a number of amino-acids in addition to those originally present in the urine.
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References
Consden, R., Gordon, A. H., Martin, A. J. P., Biochem. J., 41, 590 (1947).
Dent, C. E., Biochem. J., 41, 240 (1947).
Martin, A. J. P., and Mittlemann, R., Biochem. J., 43, 353 (1948).
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WYNN, V. A Peptide-like Contaminant of Filter Paper. Nature 164, 445 (1949). https://doi.org/10.1038/164445a0
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DOI: https://doi.org/10.1038/164445a0
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