Abstract
THE use of ammoniacal silver nitrate solution as a spraying reagent for revealing the presence of sugars on filter-paper chromatograms1 has the advantage of general application ; but it has a corresponding disadvantage in reacting with a very wide range of reducing substances other than the sugars, including various impurities commonly present in such solvents as phenol and collidine. Two-dimensional chromatograms are often rather unsatisfactory when ammoniacal silver nitrate is used, because (a) a rather large amount of the sugar mixture is needed and this increases the effect of interfering substances, (b) it is necessary to apply the spray as an aqueous solution, and unless the spraying is very rapid and uniform, the sugar spots migrate from wet to dry regions on the filter paper.
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References
Partridge, S. M., Nature, 158, 270 (1946); Biochem. J., 42, 238 (1948).
Partridge, S. M., Biochem. J., 43 Proc., xlviii (1948).
Consden, R., Gordon, A. H., and Martin, A. J. P., Biochem. J., 38, 224 (1944).
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PARTRIDGE, S. Aniline Hydrogen Phthalate as a Spraying Reagent for Chromatography of Sugars. Nature 164, 443 (1949). https://doi.org/10.1038/164443a0
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DOI: https://doi.org/10.1038/164443a0
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