Abstract
DURING some recent work, which will be described elsewhere, it was of interest to determine the Michaelis constants of succinic dehydrogenase with respect to succinic acid and succinic α — D2, α' — D2 acid (tetradeuterosuccinic acid). A number of determinations, carried out by the methods of Lineweaver and Burk1, appeared to reveal a slight difference between the Michaelis constants of the enzyme with respect to these two substrates. It was, however, desirable to obtain confirmation of this apparent difference by an independent method. The method evolved is described here since it seems to be generally applicable to problems of this type.
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References
Lineweaver, H., and Burk, D., J. Amer. Chem. Soc., 56, 658 (1934).
Haldane, J. B. S., “Enzymes” (Longmans, Green and Co., London, 1930).
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THORN, M. A Method for Determining the Ratio of the Michaelis Constants of an Enzyme with Respect to Two Substratos. Nature 164, 27–29 (1949). https://doi.org/10.1038/164027a0
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DOI: https://doi.org/10.1038/164027a0
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