Abstract
IN the original publication by Consden, Gordon and Martin1 on the application of partition chromatography on filter paper to the separation of amino-acids, the positions of these acids were determined by spraying the whole of the paper with a 0 1 or 0·05 per cent solution of ninhydrin in butanol. This procedure appears to have been adopted by subsequent workers in spite of inherent objections to the use of butanol in a fine spray in more or less close proximity to the operator. To avoid this objection, and to determine whether this stage could be entirely eliminated, a series of 'runs' has been made in which the ninhydrin has been incorporated in the moving phase.
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Consden, R., Gordon, A. H., and Martin, A. J. P., Biochem. J., 38, 224 (1944).
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NICHOLSON, D. Modified Technique for the Development of Paper Chromatograms. Nature 163, 954 (1949). https://doi.org/10.1038/163954b0
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DOI: https://doi.org/10.1038/163954b0
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