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Determination of Keto-Acids by Partition Chromatography on Filter-Paper

Abstract

THE method used by Consden, Gordon and Martin1 for the qualitative analysis of amino-acid mixtures, and by Partridge2 for sugars, employing filter-paper partition chromatography, was applied to the determination of some keto-acids. The keto-acids were characterized as their 2,4-dinitrophenyl. hydrazone derivatives ; this gave the advantage of manipulating coloured compounds, which showed their position on the paper without further development.

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References

  1. Consden, R., Gordon, A. H., and Martin, A. J. P., Biochem. J., 38, 224 (1944).

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  2. Partridge, S. M., Biochem. J., 42, 238 (1948).

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CAVALLINI, D., FRONTALI, N. & TOSCHI, G. Determination of Keto-Acids by Partition Chromatography on Filter-Paper. Nature 163, 568–569 (1949). https://doi.org/10.1038/163568a0

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