Wound Extracts and their Effects on the Growth of Hen Fibroblasts

Abstract

STERILE, healing wounds (rats) were extracted with physiological salt solution. Extracts were made from wounds of ages from 1 to 12 days. The extract was dialysed with several changes of distilled water at a low temperature in a 'Cellophane' tube. In this way the albumin and globulin fractions of the extracts could be separated. Both solutions were then brought to pH 6·5–7 and diluted to the same dry-weight concentration. The usual heart fibroblast cultures in Carrel flasks and Carrel glasses were later treated with the prepared extracts. The results are given in Fig. 1, in which the zero horizontal line represents the usual index of increase for the corresponding control culture. A minus index means growth inhibition, and a plus index represents increase of growth.