Abstract
FIBRINOGEN dissolved in 30 per cent urea solution readily consumes iodine even at 0° C. Analysis has shown that the action of iodine is twofold : part of the consumed iodine is substituted in the tyrosine rings in the fibrinogen molecule, and part of the iodine oxidizes certain of its groups. Thus as a result of the action of iodine, fibrinogens, iodinated and oxidized to various extents, can be prepared. To obtain such iodinated fibrinogens, a known amount of N/100 iodine solution was allowed to act on samples of fibrinogen dissolved in slightly alkaline 30 per cent urea solution. After various incubation times the action of the iodine was stopped by removing the remaining free iodine with thiosulphate. The urea was then dialysed off and the fibrinogen precipitated isoelectrically several times and finally dissolved in slightly alkaline water. The iodine bound to fibrinogen was then estimated. It was found that the iodine bound to the fibrinogen reached its maximum value in the first few minutes and then remained constant. This maximum value corresponds to two iodine atoms per tyrosine residue of fibrinogen. Simultaneously, the Millon reaction for phenolic groups disappeared.
Similar content being viewed by others
Article PDF
References
Laki, K., and Mommaerts, W. F. H. M., Nature, 156, 664 (1945).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
LAKI, K., MIHÁLYI, E. Action of Thrombin on Iodinated Fibrinogen. Nature 163, 66 (1949). https://doi.org/10.1038/163066a0
Issue Date:
DOI: https://doi.org/10.1038/163066a0
This article is cited by
-
Conditions for the association of the two clotting proteins of the cockroachRhyparobia (Leucophaea) maderae (Blattaria)
Journal of Comparative Physiology B (1988)
-
Inhibition of crosslinking in clotting of cockroach hemolymph (Leucophaea maderae) (blaberidae, blattaria)
Journal of Comparative Physiology B (1986)
-
Some Aspects of the Fibrinogen-Fibrin Transition
Nature (1960)
-
Action of Phenolic Substances on the Blood-clotting Effect of Bothrops atrox Venom
Nature (1950)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.