Abstract
PLEROCERCOID larvæ of Schistocephalus solidus and Ligula intestinalis, when removed aseptically from their respective fish hosts by means of a special technique, develop into sexually mature tapeworms if cultured in vitro in certain nutrient media at 40° C.1,2. The eggs, which were produced in vitro in large numbers, proved on testing to be infertile. It was tentatively suggested that infertility of such eggs was possibly due to failure of normal copulation to take place within the culture tube, although other factors were also considered.
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SMYTH, J. Development of Cestodes in vitro: Production of Fertile Eggs; Cultivation of Plerocercoid Fragments. Nature 161, 138 (1948). https://doi.org/10.1038/161138a0
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DOI: https://doi.org/10.1038/161138a0
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