Influence of Gonadal Hormones on the Serum Lipochrome and Riboflavin of the Domestic Fowl

Abstract

TRICHLORACETIC acid filtrates of serum were prepared during an investigation of the effects of gonadal hormones on the mineral metabolism of the immature pullet. Such preliminary removal of protein is essential in determining serum calcium where much vitellin or phospholipid is present, as is the case in laying birds or birds treated with Å"strogen. It was noticed that filtrates from the sera of the pullets (fourteen weeks old) were tinted a greenish-yellow colour in the case of those birds receiving heavier doses of Å"strogen, while the sera of birds not receiving Å"strogen were colourless. A direct dietary influence was excluded because the birds, which were of the same strain and hatching, had been reared together under the same conditions and had, for three weeks before the observations, received the same amounts daily of the same diet.