Abstract
DUNN et al.1,2,3 have described four different media, varying in their amino-acid contents, for the microbiological assay of amino-acids by means of the lactic organism Leuconostoc mesenteroides P.60, and consider that one of these media (medium D) is suitable for the assay of lysine and histidine. A re-investigation of medium D has shown that in our hands it is inadequate and acid production is poor. Apparently this has also been found by Dunn et al., for in their third communication3 they recommend the use of 0.05N sodium hydroxide solution instead of 0.1 N. In a fourth paper, Dunn et al.4 describe the assay of phenylalanine with a further modification of the medium, in which a still weaker standard solution (0.028 N) is used for titrating the acid produced, although in the assays the contents of six tubes were combined and titrated with 0.111 N sodium hydroxide.
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References
Dunn, M. S., Shankman, S., Camien, M. N., Frankl, W., and Rockland, L. B., J. Biol. Chem., 156, 703 (1944).
Dunn, M. S., Camien, M. N., Shankman, S., Frankl, W., and Rockland, L. B., J. Biol. Chem., 156, 715 (1944).
Dunn, M. S., Camien, M. N., Shankman, S., and Rockland, L. B., J. Biol. Chem., 159, 653 (1945).
Dunn, M. S., Shankman, S., and Camien, M. N., J. Biol. Chem., 161, 643 (1945).
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BARTON-WRIGHT, E., EMERY, W. & ROBINSON, F. Microbiological Assay of Amino-Acids with Leuconostoc mesenteroides P.60. Nature 157, 628 (1946). https://doi.org/10.1038/157628a0
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DOI: https://doi.org/10.1038/157628a0
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