Abstract
THIS book has been designed to give the graduate JL student a working description of the methods used as a routine in the study of tissue metabolism. Only methods which are likely to need the minimum equipment found normally in a biological laboratory such as a respirometer, colorimeter and centrifuge are described. In addition to details of manometric, Thunberg tube and related electrometric techniques, much information is given on the preparation of physiologically important metabolites and particularly on the preparation and estimation of the phos-phorylated carbohydrate intermediates. Methods are given for preparing animal, plant and microbial tissues and the homogenate technique is described. The wider use cf manometric methods for the estimation of particular metabolites, which with specific enzyme systems utilize or produce gases, such as the estimation of urea with urease and of succinic acid and xanthine by their oxidases, is advocated. A very extensive bibliography follows each of the sixteen chapters.
Manometric Techniques and Related Methods for the Study of Tissue Metabolism
W. W.
Umbreit
R. H.
Burris
J. F.
Stauffer
By. Chapters on Specialized Techniques by P. P. Cohen, G. A. LePage and V. R. Potter. Pp. vii + 198. (Minneapolis, Minn.: Burgess Publishing Company, 1945.) 3.50 dollars.
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Manometric Techniques and Related Methods for the Study of Tissue Metabolism. Nature 157, 606 (1946). https://doi.org/10.1038/157606c0
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DOI: https://doi.org/10.1038/157606c0