Abstract
THE findings of Bauernfeind et al.1 and of Strong and Carpenter2 suggest that the accuracy of microbiological assays for riboflavin, with Lactobacillus helveticus as the test organism, may be seriously affected by the presence of fatty acids in the extracts under investigation. We have studied the conditions under which such interference may occur using a medium freed from lipids by chloroform extraction. Our results will be reported in detail elsewhere3.
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References
Bauernfeind, J. C., Sotier, A. L., and Boruff, C. S., Indust. Eng. Chem., Anal. Ed., 14, 666 (1942).
Strong, F. M., and Carpenter, L. E., ibid., 14, 909 (1942).
Kodicek, E., and Worden, A. N., Biochem. J. (in the Press).
Snell, E. E., and Strong, F. M., Indust. Eng. Chem., Anal. Ed., 11, 346 (1939).
Snell, E. E., and Strong, F. M., Univ. Texas Publication No. 4137 (1941).
Barton-Wright, E. C., and Booth, R. G., Biochem. J., 37, 25 (1943).
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KODICEK, E., WORDEN, A. Effect of Unsaturated Fatty Acids upon the Growth of Lactobacillus helveticus and other Gram-positive Bacteria. Nature 154, 17–18 (1944). https://doi.org/10.1038/154017b0
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DOI: https://doi.org/10.1038/154017b0
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