Abstract
IT is usually assumed that the depth of focus, t, of a well-corrected microscope objective is given by one or other of the following formulæ: where N is the refractive index of the medium through which the light travels, the wave-length of the light used, and N.A. the numerical aperture of the objective.
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References
J. Opt. Soc. America, 27, No. 11 (Nov. 1937).
Beadle, J. Sci. Inst., 16, No. 8, 262.
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BEADLE, D. Depth of Focus of Microscope Objectives. Nature 145, 1018–1019 (1940). https://doi.org/10.1038/1451018b0
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DOI: https://doi.org/10.1038/1451018b0
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