Dissociation of Ovalbumin in Urea Solvent

Abstract

THE physical molecule in solution is that unit which will acquire from thermal vibrations an average of 1/2 kT ergs of energy for each of three translational degrees of freedom. It is becoming increasingly evident that the weights of protein molecules defined in this way may change with solvent temperature, dielectric constant, protein or salt concentration, and other factors. Numerous studies from this Laboratory have demonstrated the effect of change of pH of the solvent medium in the dissociation and association of the molecules comprising the fundamental component.