Abstract
IN a recent communication, Klar1 has questioned the results of experiments which seem to indicate that diplogen exerts a stimulative effect. He would attribute any such increase in growth as has been noted to the presence of organic impurity rather than to the heavy isotope of hydrogen. The investigations of Barnes2, Barnes and Larson3, Richards4, and Meyer5 have shown that such stimulation does occur and it has been attributed to the presence of the D atom. I was aware that there was a certain amount of inorganic, and possibly organic, impurity in the Ohio-water used, approximately 0.04 per cent or less, but am of the opinion that the method of purification by a careful double- and triple-distillation in pyrex glassware removed any such impurity. The investigations reported here seem to substantiate this view.
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References
Klar, NATURE, 134, 104, July 21, 1934.
Barnes, J. Amer. Chem. Soc., 55, 4332; 1933. Amer. J. Bot., 20, 679; 1933. Science, 79, 370; 1934.
Barnes and Larson, J. Amer. Chem. Soc., 55, 5059; 1933. NATURE, 133, 873, June 9, 1934.
Richards, Amer. J. Bot., 20, 679; 1933. J. Bact., 28, 289; 1934.
Meyer, Science, 79, 210; 1934. J. Tenn. Acad. Sci., 9, No. 4, 1934 (in press).
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MEYER, S. Alleged Stimulation of Moulds by Paraffin in Heavy Water. Nature 134, 665 (1934). https://doi.org/10.1038/134665a0
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DOI: https://doi.org/10.1038/134665a0
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