Abstract
THE method for determining the concentration gradient in the ultra-centrifuge cell has to be chosen differently according to the different properties of the solution centrifuged, and the different purposes of the research. For substances with suitable absorption in the visible or ultra-violet wave-length region, the original photographic-photometric method of The Svedberg1 has certain advantages, particularly in the case of solutions containing two or more components which possess absorption bands at different wave-lengths. It is then often possible to get the concentration curve for each component by taking photographs of the cell with light of different wave-lengths.
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References
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LAMM, O. A New Method for determining the Concentration Gradient in the Ultra-Centrifuge Cell. Nature 132, 820–821 (1933). https://doi.org/10.1038/132820a0
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DOI: https://doi.org/10.1038/132820a0
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