Abstract
THE importance of always omitting lipide solvents from fixatives for mitochondria has, I think, been exaggerated. I find that mitochondria are readily demonstrable in the liver of the newt and the kidney of the mouse even after fixation with Carnoy's alcohol-chloroform-acetic, which should certainly remove all lipides. Bouin's fluid preserves the mitochondria in the liver of the newt as well as the standard mito-chondrial fixatives. When in Dalmatia in the summer I put the testes of some Hemiptera (Syromaetes marginatus) into Bouin's fluid and left them in it until my return to England. On sectioning them I found the filamentous mitochondria of the primary spermatocytes well preserved.
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Baker, J. R., NATURE, 130, 134, July 23, 1932.
Cnampy, C., Arch. d' Anat. micr., 13, p. 55; 1911.
Romeis, B., Arch. mikr. Anat., 81, p. 129; 1913.
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BAKER, J. Fixation of Mitochondria. Nature 130, 741 (1932). https://doi.org/10.1038/130741a0
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DOI: https://doi.org/10.1038/130741a0
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